SIH Medium Size:1kg Suspend 19

Suspend 19

1% Store at 2 - 8°C in dark

2 at 25°C Suspend 28 gram powdered medium in 1 litre distilled water

2 at 25°C Suspend 40 gram powdered medium in 1 litre distilled water

DNA replication inhibitor causing deoxyribonucleotide depletion

SIH Medium Size:1kg Suspend 19SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic